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After some searching on other forums it seems the sketch upload error is just a communications error. Is there any reason why I have previously been able to upload sketches to my board and send output to the serial monitor, but now I cannot? Could it be a driver issue?
I did not install the FTDI virtual COM port (VCP) driver because when I first connected the Mayfly to my PC it was recognized… my IT dept said that I already had the necessary driver installed. Perhaps this is not the case?